Targeting Mitochondrial Protein Expression as a Future Approach for Cancer Therapy

Extensive metabolic remodeling is a fundamental feature of cancer cells. Although early reports attributed such remodeling to a loss of mitochondrial functions, it is now clear that mitochondria play central roles in cancer development and progression, from energy production to synthesis of macromolecules, from redox modulation to regulation of cell death. Biosynthetic pathways are also heavily affected by the metabolic rewiring, with protein synthesis dysregulation at the hearth of cellular transformation. Accumulating evidence in multiple organisms shows that the metabolic functions of mitochondria are tightly connected to protein synthesis, being assembly and activity of respiratory complexes highly dependent on de novo synthesis of their components. In turn, protein synthesis within the organelle is tightly connected with the cytosolic process. This implies an entire network of interactions and fine-tuned regulations that build up a completely under-estimated level of complexity. We are now only preliminarily beginning to reconstitute such regulatory level in human cells, and to perceive its role in diseases. Indeed, disruption or alterations of these connections trigger conditions of proteotoxic and energetic stress that could be potentially exploited for therapeutic purposes. In this review, we summarize the available literature on the coordinated regulation of mitochondrial and cytosolic mRNA translation, and their effects on the integrity of the mitochondrial proteome and functions. Finally, we highlight the potential held by this topic for future research directions and for the development of innovative therapeutic approaches

MORAL MATURATION AND MORAL CONATION: A CAPACITY APPROACH TO EXPLAINING MORAL THOUGHT AND ACTION

We set out to address a gap in the management literature by proposing a framework specifying the component capacities organizational actors require to think and act morally. We examine how moral maturation (i.e., moral identity, complexity, and metacognitive ability) and moral conation (i.e., moral courage, efficacy, and owner- ship) enhance an individual\u27s moral cognition and propensity to take ethical action. We offer propositions to guide future research and discuss the implications of the proposed model for management theory an

Application of the LEPS technique for Quantitative Precipitation Forecasting (QPF) in Southern Italy: a preliminary study

International audienceThis paper reports preliminary results for a Limited area model Ensemble Prediction System (LEPS), based on RAMS (Regional Atmospheric Modelling System), for eight case studies of moderate-intense precipitation over Calabria, the southernmost tip of the Italian peninsula. LEPS aims to transfer the benefits of a probabilistic forecast from global to regional scales in countries where local orographic forcing is a key factor to force convection. To accomplish this task and to limit computational time in an operational implementation of LEPS, we perform a cluster analysis of ECMWF-EPS runs. Starting from the 51 members that form the ECMWF-EPS we generate five clusters. For each cluster a representative member is selected and used to provide initial and dynamic boundary conditions to RAMS, whose integrations generate LEPS. RAMS runs have 12-km horizontal resolution. To analyze the impact of enhanced horizontal resolution on quantitative precipitation forecasts, LEPS forecasts are compared to a full Brute Force (BF) ensemble. This ensemble is based on RAMS, has 36 km horizontal resolution and is generated by 51 members, nested in each ECMWF-EPS member. LEPS and BF results are compared subjectively and by objective scores. Subjective analysis is based on precipitation and probability maps of case studies whereas objective analysis is made by deterministic and probabilistic scores. Scores and maps are calculated by comparing ensemble precipitation forecasts against reports from the Calabria regional raingauge network. Results show that LEPS provided better rainfall predictions than BF for all case studies selected. This strongly suggests the importance of the enhanced horizontal resolution, compared to ensemble population, for Calabria for these cases. To further explore the impact of local physiographic features on QPF (Quantitative Precipitation Forecasting), LEPS results are also compared with a 6-km horizontal resolution deterministic forecast. Due to local and mesoscale forcing, the high resolution forecast (Hi-Res) has better performance compared to the ensemble mean for rainfall thresholds larger than 10mm but it tends to overestimate precipitation for lower amounts. This yields larger false alarms that have a detrimental effect on objective scores for lower thresholds. To exploit the advantages of a probabilistic forecast compared to a deterministic one, the relation between the ECMWF-EPS 700 hPa geopotential height spread and LEPS performance is analyzed. Results are promising even if additional studies are required

Predictability of intense rain storms in the Central Mediterraneanbasin: sensitivity to upper-level forcing

International audienceThis study investigates the sensitivity of a moderate-intense storm that occurred over Calabria, southern Italy, to upper-tropospheric forcing from a Potential Vorticity (PV) perspective. A prominent mid-troposheric trough can be identified for this event, which occurred between 22?24 May 2002, and serves as the precursor agent for the moderate-intense precipitation recorded. The working hypothesis is that the uncertainty in the representation of the upper-level disturbance has a major impact on the precipitation forecast and we test the hypothesis in a two-step approach. First, we examine the degree of uncertainty by comparing five different scenarios in a Limited area model Ensemble Prediction System (LEPS) framework which utilizes the height of the dynamical tropopause as the discriminating variable. Pseudo water vapour images of different scenarios are compared to the corresponding METEOSAT 7 water vapour image at a specific time, antecedent to the rain occurrence over Calabria, in order to evaluate the reliability of the different precipitation scenarios simulated by the LEPS. Second, we examine the impact of upper tropospheric PV variations on precipitation by comparing model simulations with slightly different initial PV fields. Initial velocity and mass fields in each case are balanced with the chosen PV perturbation using a PV inversion technique. The results of this study support the working hypothesis

Robotic-assisted surgery for excision of an enlarged prostatic utricle

INTRODUCTION: Prostatic utricle is a rare malformation arising from incomplete regression of the Mullerian ducts. Diagnosis is easily made but management may be challenging. The minimally invasive approach has so far been considered the gold standard for surgical treatment. Many endoscopic and surgical procedures have been described for removal, but to date only few cases of robot-assisted procedures have been mentioned in the literature and there are no reports of redos. PRESENTATION OF THE CASE: We report the case of a giant prostatic utricle cyst successfully treated with robotic-assisted surgery two years after an unsuccessful first attempt at laparoscopic excision. No relapse was found at one year follow up. DISCUSSION: We were able to excise a retro-vescical structure in spite of adhesions caused by the previous surgery and the very large size of the diverticulum thanks to the high magnification and 3-D visualization available in robotic assisted laparoscopy. CONCLUSION: Robot assisted laparoscopy should be considered an advantageous technique for the treatment of prostatic utricle

Efeitos do Programa Escola de Postura e Reeducação Postural Global sobre a amplitude de movimento e níveis de dor em pacientes com lombalgia crônica

ResumoObjetivoComparar os efeitos do programa escola de postura (PEP) e reeducação postural global (RPG) sobre níveis de dor e amplitude de movimento em pacientes com lombalgia crônica.MétodoA amostra foi dividida em 3 grupos de 10 sujeitos: grupo submetido ao tratamento através do PEP (idade: 46.30±8.50 anos); grupo submetido ao tratamento através da RPG (idade: 43.60±10.93 anos) e grupo controle (idade: 44.30±10.68 anos). As intervenções foram realizadas em 10 sessões. Para avaliação do quadro álgico foi utilizada a escala subjetiva de dor CR10 de Borg. Para a análise da amplitude de movimento empregou‐se o protocolo de goniometria LABIFIE para os movimentos de extensão coxofemoral (ECF) e flexão da coluna lombar (FCL).ResultadosO teste de Wilcoxon mostrou uma redução nos escores dos níveis de dor nos grupos PEP e RPG (p<0.0001) do pré para o pós‐tratamento. As comparações intergrupos, através do teste de Kruskal‐Wallis, apresentaram diminuição dos níveis de dor para o PEP (p<0.0001) e o RPG (p<0.0001) quando comparados ao CG no pós‐tratamento. A ANOVA com medidas repetidas revelou um aumento na amplitude do movimento para o PEP (ECF: p=0.006; FCL: p=0.002) e RPG (ECF: p=0.034; FCL: p=0.011) do pré para o pós‐tratamento. As comparações intergrupos apresentaram maiores amplitudes de movimento para o PEP (ECF: p=0.006; FCL: p=0.018) e RPG (EQ: p=0.019; FCL: p=0.020) quando comparados ao CG no pós‐tratamento. Não houve diferenças significativas entre o PEP e RPG.ConclusãoOs tratamentos PEP e RPG se mostraram eficientes para redução da lombalgia crônica.AbstractObjectiveTo compare the effects of school‐based exercise program of posture (SPP) and global postural reeducation (GPR) on pain levels and range of motion in patients with chronic low back pain.MethodThe sample was divided into three groups of 10 subjects: group treated by SPP (age: 46.30±8.50 years) group subjected to treatment by GPR (age: 43.60±10.93 years) and control group (age: 44.30±10.68 years). The interventions were performed in 10 sessions. For assessment of pain was used pain subjective scale CR10‐Borg. For the analysis of range of motion, we used the protocol to goniometry LABIFIE in the movements of hip extension (HE) and flexion of the lumbar spine (FLS).ResultsThe Wilcoxon test showed a reduction in levels of pain scores in groups SPP and GPR (p<0.0001) of pre to post‐treatment. Comparisons between groups by the Kruskal‐Wallis test showed decreased levels of pain for SPP (p<0.0001) and GPR (p<0.0001) when compared to CG post‐treatment. Repeated measures ANOVA revealed an increase in range of motion for the SPP (HE: p=0.006; FLS: p=0.002) and GPR (HE: p=0.034; FLS: p=0.011) of pre to post‐treatment. Comparisons between groups showed greater range of motion for the SPP (HE: p=0.006; FLS: p=0.018) and GPR (HE: p=0.019; FLS: p=0.020) when compared to CG post‐treatment. There were no significant differences between the SPP and GPR.ConclusionsSPP and GPR treatments were effective for reducing chronic low back pain

Unlocking the mask: A look at the process by which authentic leaders impact follower attitudes and behaviors

The conceptual and empirical links between authentic leadership and follower attitudes, behaviors, and performance outcomes have not been fully developed. Although we have a number of articles developing the theory of authentic leadership and testing propositions that will appear in a forthcoming special issue of The Leadership Quarterly (Vol. 16, Issue 3, 2005), the focus of this article is to provide some of the initial foundation work for the broader theoretical framework of how authentic leaders influence follower attitudes, behaviors, and performance. Here, we draw from positive organizational behavior, trust, hope, emotion, identification, and identity theories to describe the processes by which authentic leaders exert their influence on followers’ attitudes and behaviors. Research propositions based on the proposed theoretical model and implications for future theory building and research are presented

L’ambiente marino costiero: aspetti e tutela. Progetto formativo di Alternanza Scuola Lavoro 2016‐2019 Liceo Scienze Applicate

Il percorso formativo consente di acquisire nozioni teoriche e pratiche sulle moderne tecniche (strumentali e metodologiche) di investigazione del “datum” geologico finalizzate allo studio multidisciplinare dell’ambiente marino‐costiero, con particolare riguardo alle ricerche sperimentali che l’Istituto IAMC ha condotto e conduce nel Golfo di Napoli. Vengono trattate anche tematiche di gestione del sistema sicurezza e qualità con particolare riguardo alle attività lavorative di ricerca (acquisizione, elaborazione e restituzione del dato),nonché indicazioni di procedure gestionali di progetto finalizzate al corretto utilizzo della risorsa umana e strumentale